Sean Ling
Brown University
Monday, November
9, 2009
4:00 pm in SPL 57
Single-molecule biophysics with solid-state nanopores
Abstract: In this talk, I'll discuss recent advances in the field of nanopore biophysics. Inspired by the original proposal of nanopore DNA sequencing using biological nanopores, solid-state nanopores have been developed recently using silicon technology as a more robust alternative for single-molecule analysis. Because of their tunable size in solid-state nanopores, there are intense interests in using nanopores as a one-dimensional scanner for hybridization probes on single DNA molecules. I will discuss our recent experiments in which we show that a solid-state nanopore can be used to scan over a ~400-base single-stranded DNA molecule with two ~ 4nm long double-stranded segments. This is a key milestone to achieving DNA sequencing using the SBH (sequencing-by-hybridization) method.
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